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Monday, August 10, 2020 | History

1 edition of Enzymes in nonaqueous solvents found in the catalog.

Enzymes in nonaqueous solvents

Evgeny N. Vulfson

Enzymes in nonaqueous solvents

methods and protocols

by Evgeny N. Vulfson

  • 32 Want to read
  • 8 Currently reading

Published by Humana Press in Totowa, NJ .
Written in English

    Subjects:
  • Enzymes,
  • Biotechnology,
  • Nonaqueous solvents

  • Edition Notes

    Includes bibliographical references and index.

    Statementedited by Evgeny N. Vulfson, Peter J. Halling, and Herbert L. Holland
    SeriesMethods in biotechnology -- 15, Methods in biotechnology -- 15.
    Classifications
    LC ClassificationsTP248.65.E59 E624 2011
    The Physical Object
    Paginationxix, 679 p. :
    Number of Pages679
    ID Numbers
    Open LibraryOL25559529M
    ISBN 101617372560
    ISBN 109781617372568
    OCLC/WorldCa823504686

    Enzymes in Nonaqueous Solvents: Methods and Protocols (Methods in Biotechnology) by Editor-Evgeny N. Vulfson. Humana Press, Hardcover. Good.   Enzymes have evolved to function under aqueous conditions and may not exhibit features essential for biocatalytic application, such as the ability to function in high concentrations of an organic solvent. Consequently, protein engineering is often required to tune an enzyme for catalysis in non‐aqueous solvents.

      The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media. Studies over the past 15 years have revealed not. Closing a gap in the literature, this comprehensive book examines and discusses different non-aqueous systems from organic solvents to ionic liquids for .

      Chen, K., Arnold, F. Enzyme Engineering for Nonaqueous Solvents: Random Mutagenesis to Enhance Activity of Subtilisin E in Polar Organic Media. Nat Biotechnol 9, – (). https://doi Cited by: "Organic Synthesis with Enzymes in Non-Aqueous Media provides an excellent overview of the fundamental principles and utility of biocatalysts in organic transformations and represents a comprehensive reference guide for both organic chemists and enzymologists interested in the field."Journal of the American Chemical Society.


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Enzymes in nonaqueous solvents by Evgeny N. Vulfson Download PDF EPUB FB2

In Enzymes in Nonaqueous Solvents: Methods and Protocols, leading chemists, biochemists, biotechnologists, and process engineers summarize for the first time a wide range of methods for executing enzymatic transformations under nonaqueous : Hardcover. In Enzymes in Nonaqueous Solvents: Methods and Protocols, leading chemists, biochemists, biotechnologists, and process engineers summarize for the first time a wide range of methods for executing enzymatic transformations under nonaqueous conditions.

In recent years, enzymatic catalysis in organic solvents-as opposed to aqueous solutions-has gained considerable attention as a powerful new approach to the preparation of natural products, pharmaceuticals, fine chemicals, and food ingredients.

In Enzymes in Nonaqueous Solvents: Methods and Protocols, leading chemists, biochemists, biotechnologists, and process 5/5(1).

Introduction. In recent years, enzymatic catalysis in organic solvents-as opposed to aqueous solutions-has gained considerable attention as a powerful new approach to the preparation of natural products, pharmaceuticals, fine chemicals, and food ingredients. In Enzymes in Nonaqueous Solvents: Methods and Protocols, leading chemists, biochemists, biotechnologists, and process engineers summarize for the first time a wide range of methods for executing enzymatic.

Enzymes in Nonaqueous Solvents: Methods and Protocols Evgeny N. Vulfson In Enzymes in Nonaqueous Solvents: Methods and Protocols, leading chemists, Enzymes in nonaqueous solvents book, biotechnologists, and process engineers summarize for the first time a wide range of methods for executing enzymatic transformations under nonaqueous conditions.

"Organic Synthesis with Enzymes in Non-Aqueous Media provides an excellent overview of the fundamental principles and utility of biocatalysts in organic transformations and represents a comprehensive reference guide for both organic chemists and enzymologists interested in the field."Journal of the American Chemical SocietyPrice: $ Water – A Unique and Necessary Solvent for Enzymatic Catalysis.

Enzyme Activation in Nonaqueous Media. Salt-Activated Enzymes. Conclusions. Part Two Biocatalysis in Neat Organic Solvents – Synthetic Applications 4 Exploiting Enantioselectivity of Hydrolases in Organic Solvents (Hans-Erik Högberg).

Introduction. Enzymes in nonaqueous solvents book reversal of hydrolytic enzymes and thus avoids the use of the expensive cofactors or activated substrates that are required for their synthetic count- parts. This item ships from multiple locations. Your book may arrive from Roseburg,OR, La Vergne,TN.

Hardcover. Read Enzymes in Nonaqueous Solvents: Methods and Protocols Online. "Organic Synthesis with Enzymes in Non-Aqueous Media provides an excellent overview of the fundamental principles and utility of biocatalysts in organic transformations and represents a comprehensive reference guide for both organic chemists and.

enzymes catalyzing reactions in the absence of water, i.e. in non-aqueous media. Addition of organic solvents was done either to precipitate enzymes or to study denaturation. Thus, the study of enzyme action in organic solvents is a compara- tively recent aspect of enzymology.

Singer () reviewed theCited by: Enzymes in Nonaqueous Solvents: Methods and Protocols, edited by Evgeny N. Vulfson, Peter J. Halling, and Herbert L. Holland, Food Microbiology Protocols, edited byJ. Spencer and Alicia Leonor Ragout de Spencer, Supercritical Fluid Methods and Protocols, edited by John R.

Williams and Anthony A. Clifford,   Book Title. Organic Synthesis with Enzymes in Non-Aqueous Media. Additional Information. How to Cite. Adlercreutz, P. () Fundamentals of Biocatalysis in Neat Organic Solvents, in Organic Synthesis with Enzymes in Non-Aqueous Media (eds G.

Carrea and S. Riva), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi:. In nature, the vast majority of enzyme-catalyzed reactions are carried out in water. Traditionally, enzymic catalysis in organic synthesis is carried out in aqueous Enzymes in Nonaqueous Solvents | SpringerLinkCited by:   Control of Enzyme Activity in Nonaqueous Solvents Peter J.

Halling Salt-Induced Activation of Enzymes in Organic Solvents: Optimizing the Lyophilization Time and Water Content Michael T. Ru, Jonathan S. Dordick, Jeffrey A. Reimer, and Douglas S. Clark Imprinting Enzymes for Use in Organic Media Joseph O.

Rich and Jonathan S. DordickPrice: $ Closing a gap in the literature, this comprehensive book examines and discusses different non-aqueous systems from organic solvents to ionic liquids for synthetic applications, thus opening the door to new successful methods for biocatalytic reactions.

It gathers into one handy source the information otherwise widely spread throughout the literature, combining useful. In nonaqueous solutions, reactions such as transesterification or ammonolysis can be performed.

Enzymes are clearly active in organic solvents which appears to contradict our central concepts of protein stability. Two reasons could could explain this stability. It is possible that from a thermodynamic view, the enzyme is stable in organic solvents. Enzymes in Nonaqueous Solvents | Enzymatic catalysis has gained considerable attention in recent years as an efficient tool in the preparation of natural products, pharmaceuticals, fine chemicals, and food ingredients.

Key attributes of reactions in nonaqueous solvents ; 3. Kinds of nonaqueous solvents used for biocatalysis ; 4.

Solubility of enzymes, substrates, and products in nonaqueous solvents ; 5. Stability of enzymes in nonaqueous solvents ; 6. Selection of nonaqueous solvents for a desired reaction ; 7. Examples of reactions in organic solvents ; 8.

The double mutant QR+NS is 10 times more active than the wild‐type enzyme in 20% (v/v) DMF and twice as stable in 40% DMF. Although the effects of single mutations can be impressive, a practical strategy for engineering enzymes that function in nonaqueous solvents will most likely require multiple changes in the amino acid by: The reduction of enzyme activity at higher levels of hydration was suggested (Affleck et al., ) to be explained by conformation changes that could alter the active site, consistent with the observation that enzymes display much more native structure in pure organic solvents than in aqueous-organic mixtures (Griebenow and Klibanov, ).Cited by:.

Engineering enzymes for non-aqueous solvents Frances H. Arnold Enzymes may be redesigned to permit catalysis in non-aqueous sol- vents by engineering their amino acid sequences, thereby altering their physical and chemical properties to suit the new solvent en- .Enzyme engineering for nonaqueous solvents.

II. Additive effects of mutations on the stability and activity of subtilisin E in polar organic media Enzyme engineering for nonaqueous solvents. II. Engineering enzymes with novel reaction modes promises to expand the applications of biocatalysis in chemical synthesis and will enhance our Cited by: ISBN: OCLC Number: Description: xix, pages: illustrations ; 24 cm.

Contents: Part I Control of Enzyme Activity in Nonaqueous Solvents Peter J. Halling Salt-Induced Activation of Enzymes in Organic Solvents: Optimizing the Lyophilization Time and Water Content Michael T.

Ru, Jonathan S. Dordick, Jeffrey A. Reimer, and Douglas S. Clark Imprinting Enzymes.